Insertion junction: CLIP2.048870_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.048870
Chromosome:chromosome 12
Location:8756858
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g547350 CYG31 Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACGACACGACACGACATGCCGCATGAACGTTCACTCAATCCCTGAAGT
Internal bar code:AAACGATTTTTGTAATTAATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1346
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCCAGGAGAGGTCTGCAT
Suggested primer 2:CAGCCCCATCCGATAGACAC