Insertion junction: CLIP2.048896_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.048896
Chromosome:chromosome 7
Location:4856169
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g346300 (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGTGCAGTCGTTCTGCGTGGTGTGGTGCAGGATGGGTAGCGGCGTGT
Internal bar code:ATCCCCCGTCATAGCCTTAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:2523
LEAP-Seq percent confirming:98.4375
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:64

Suggested primers for confirmation by PCR

Suggested primer 1:AGAACGAATTGCCGCAAACC
Suggested primer 2:GCCGACTTGAAAGAGACGGA