Insertion junction: CLIP2.048900_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.048900
Chromosome:chromosome 16
Location:36249
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g696000 (1 of 2) 2.7.7.23 - UDP-N-acetylglucosamine diphosphorylase / UDP-N-acetylglucosamine pyrophosphorylase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCTCACCGCCGGCGAGGCGAAGTCCGACAGCCGGCGCTCCAGCCGGT
Internal bar code:CATGCCATTTGTCGAGTTATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:402
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAACCACGTTGGACCTGC
Suggested primer 2:ATGGACGCATACAGGTGCAA