| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048902 |
| Chromosome: | chromosome 11 |
| Location: | 1506299 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468900 | FAP404 | Flagellar Associated protein 404 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGGTGTTGCGGCGTGTGGTTTGGGGGTTGCGGCTTGTGGGTTGCGGT |
| Internal bar code: | ATTGAAGGACCATGTCTAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3193 |
| LEAP-Seq percent confirming: | 56.4103 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGACACAATGCAGCTGC |
| Suggested primer 2: | GAGGCAGCACAATAGTGGGT |