| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.048921 |
| Chromosome: | chromosome 3 |
| Location: | 306913 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144484 | CDPK10 | Calcium/calmodulin-dependent protein kinase; (1 of 4) PTHR24349//PTHR24349:SF121 - SERINE/THREONINE-PROTEIN KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCATCGCGGCTTGCCTTGTGAGGCCCAGCAGTCATCCAGCACCCTCGCA |
| Internal bar code: | CCGTGCCGTTCGAATCGTGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2243 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTCATCCACGACATCGG |
| Suggested primer 2: | GGCTCATACTTGCTGCATGC |