| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.048926 |
| Chromosome: | chromosome 15 |
| Location: | 408718 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635600 | NPC1,EGD2 | (1 of 1) K03626 - nascent polypeptide-associated complex subunit alpha (EGD2, NACA); GAL4 DNA-binding enhancer protein 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAACGCGGAGTACTCAGTATATTTACCTCTCGGGCCGTGAGCTCCCAGC |
| Internal bar code: | TTTCTAACTTCATGATGTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1611 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCTCCTCATGCTCCT |
| Suggested primer 2: | ATCACAGGGCAGCAAGTCTC |