| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.048939 |
| Chromosome: | chromosome 16 |
| Location: | 3558705 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669193 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGACCCAGCCGGCGGCACAGTCGCGGCTGGCGGAGCGGGAGGCAGAGT |
| Internal bar code: | TAATACACGTTATGCTGTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1608 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCCAGGAAGATGTGCGT |
| Suggested primer 2: | TATCTGGCGCCCATGTGTTT |