| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048951 |
| Chromosome: | chromosome 6 |
| Location: | 6554748 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g800721 | (1 of 1) K17582 - antigen KI-67 (MKI67) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCCTGCACCGCCGTCGCCTGCGCCTCCCTCGCCGGAGCCGCCCAGCC |
| Internal bar code: | TAATATGGAAGGGTCAACCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3034 |
| LEAP-Seq percent confirming: | 67.8571 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTCAGGAGATGGAGGAG |
| Suggested primer 2: | GAGCCTCCTAGCCCTGCA |