| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048954 |
| Chromosome: | chromosome 12 |
| Location: | 8420659 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550200 | SMM44 | (1 of 2) 2.1.1.9 - Thiol S-methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCTGGTCAAATGTGAGCCAGGGCCCGCGGTCCTCCACTCGCGAGGGCC |
| Internal bar code: | ACACGTACAGGTTCACCATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2025 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGTCGTGGCTTCCTCTC |
| Suggested primer 2: | CCAATCAGATGCAGCACACG |