| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.048957 |
| Chromosome: | chromosome 4 |
| Location: | 2776729 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223700 | (1 of 2) K07735 - putative transcriptional regulator (algH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAACTGAACACGACCCGCACATTTGTCGAAAAGACTCTCACTACTAACA |
| Internal bar code: | TGACAAGGCAATGGGAGGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1354 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGGCTCGGACATCAATT |
| Suggested primer 2: | AAGGCATGGCGATGTGGTAA |