Insertion junction: CLIP2.048988_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.048988
Chromosome:chromosome 10
Location:2433456
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g435300 AAP1 Aspartyl aminopeptidase-like protein; (1 of 1) 3.4.11.21 - Aspartyl aminopeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGGGCGCGCACACGGACTCGCCCTGCCTCAAGCTCAAGCCCGTGTCGC
Internal bar code:TAGTCTTATCGTATCCATATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1492
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AATCCCTTACGGCCCAGTTG
Suggested primer 2:CAACGAAAACGGGAGTGCAG