| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048992 |
| Chromosome: | chromosome 16 |
| Location: | 1040297 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649250 | (1 of 4) PF13911 - AhpC/TSA antioxidant enzyme (AhpC-TSA_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACGTGTGTGGAGACGCATCAGCACGGACGCATGAGGAACAAAGCACA |
| Internal bar code: | GGGCTTTAAAGGACTGAGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2320 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATCCCCGCGCTTTAGTTT |
| Suggested primer 2: | GGCTTGGAACATGTAGGGCT |