Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.049000 |
Chromosome: | chromosome 14 |
Location: | 2481443 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g624000 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGGCGGCGGGGGCGGCGGTGCCAGGCCATGCGGCGGTCGCAGGTTCA |
Internal bar code: | GTCGCCGGGAGGTGCCTTGCGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1634 |
LEAP-Seq percent confirming: | 50.0 |
LEAP-Seq n confirming: | 29 |
LEAP-Seq n nonconfirming: | 29 |
LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCATCAACTTTTGTGGCCG |
Suggested primer 2: | CATAATTGGAGCCGCAGCAC |