Insertion junction: CLIP2.049016_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.049016
Chromosome:chromosome 13
Location:1958580
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g576350 PFP2 Prefoldin-family protein; (1 of 1) K17560 - unconventional prefoldin RPB5 interactor 1 (URI1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGCGGCGGCGGCGGGTGTCGTTTGCCGACGGATCGGCGCCGGGGCAGGA
Internal bar code:CGACCTAGGAATGACCAGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:4168
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:TCACAGTGGCTTTCCTCGAC
Suggested primer 2:CTGCGCATGTCACTCACAAC