| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049025 |
| Chromosome: | chromosome 10 |
| Location: | 2121862 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433150 | (1 of 1) PF01344//PF13415 - Kelch motif (Kelch_1) // Galactose oxidase, central domain (Kelch_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGCCTCACCCCGCTCTGTCCCGCTGGTGCCCTCCCGGCCATCCTCA |
| Internal bar code: | GCGACCGTTGTAAGTGAACGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 105 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGAACGGAATGACGCGG |
| Suggested primer 2: | TGAGTACCCACGCTCAACAC |