Insertion junction: CLIP2.049030_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.049030
Chromosome:chromosome 12
Location:4279633
Confidence (%):40
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAGACGCGCCCGACGAACTTTCCGTGTTTACATGCTAATAGAGCTCGC
Internal bar code:ACTGGTTCACTAGACAAGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1600
LEAP-Seq percent confirming:16.6667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACTACTGGTCCTTGCGTGTG
Suggested primer 2:AAAGTTCAGGGTCGGTCGTC