| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049035 |
| Chromosome: | chromosome 6 |
| Location: | 1991732 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g800621 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCACCGAACCCGAGCGGGGCGACACGGAGAACAGCAGATCACCCGCCG |
| Internal bar code: | TGTTTTGGGATCGGTTCCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3790 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGAGATTGCCCAGGACTT |
| Suggested primer 2: | AGCCTGCTCAATCGTTCACA |