Insertion junction: CLIP2.049049_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.049049
Chromosome:chromosome 16
Location:1697384
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g654700 PHC61 Pherophorin-chlamydomonas homolog 61; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCGTGCCCTCCGCAACCCCGTGGGCTGGGACGTGCCCTACAGGTAAG
Internal bar code:GTCCTAGTGTCGTGTCGTTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:127
LEAP-Seq percent confirming:14.2857
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CTAGCACCACCACTCGAAGG
Suggested primer 2:CTGTGGGTAAGCGCAGGTAA