| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049055 |
| Chromosome: | chromosome 12 |
| Location: | 4838982 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g523500 | DER1 | DERLIN-1 involved in ER-associated degradation system; (1 of 2) K13989 - Derlin-2/3 (DERL2_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTCGGGCCCACCTCCCACCCGCCCTGTCCCACGCCTGCCGCCTCGCCG |
| Internal bar code: | GAATTGGAGGTCCGTCTGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 868 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCGCTGTTCATCGTCAC |
| Suggested primer 2: | GGGTGGGACAGTGAGAAGTG |