| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.049060 |
| Chromosome: | chromosome 9 |
| Location: | 1499727 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399550 | (1 of 1) PTHR10857//PTHR10857:SF11 - COPINE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACACATGCTCCTCACACATGCATAGGGCCATCGCCGACCACTACGCC |
| Internal bar code: | CTTTCGTAGTCTAAAAAGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACATCAACGGCTAACCC |
| Suggested primer 2: | GCCAAGTCAACACCGGAAAC |