| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049061 |
| Chromosome: | chromosome 1 |
| Location: | 4753517 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g033000 | FAP334 | (1 of 2) IPR000157//IPR016024 - Toll/interleukin-1 receptor homology (TIR) domain // Armadillo-type fold; Flagellar Associated Protein 334 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGGGAGGCATGGAGGAGGGGGAGAGGCGCGCGCGGGAGGGGGGTGC |
| Internal bar code: | AACCAGTGAGGGTCGAGTACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1775 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTGTGAGTGTGATGAGC |
| Suggested primer 2: | CATCTACTCTCGCACCGGTC |