| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049076 |
| Chromosome: | chromosome 17 |
| Location: | 1584164 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707300 | PGA3 | Phospholipid/glycerol acyltransferase; (1 of 1) PTHR23063:SF1 - 1-ACYLGLYCEROPHOSPHOCHOLINE O-ACYLTRANSFERASE 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCCACAGCCCCAACTCCCCCTTCCGTTCACCACCCCCTTTTACCTCC |
| Internal bar code: | CAGGATTCCACTAACGGGAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2324 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGTACTTGAGCACCAGC |
| Suggested primer 2: | TCAAGTGGGTGTACGTGAGC |