| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.049079 |
| Chromosome: | chromosome 3 |
| Location: | 2428601 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158800 | (1 of 1) 3.1.2.12 - S-formylglutathione hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGCGAGGCGGCGGGCGAGGCGGCGGTTGACACGGATGGCGAGACAG |
| Internal bar code: | TGAACAAGGATGCAAGGCTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5065 |
| LEAP-Seq percent confirming: | 92.5373 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCGTCAATAAGCAGTTCG |
| Suggested primer 2: | CGACGCCAACAAAAGCTCAA |