Insertion junction: CLIP2.049088_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.049088
Chromosome:chromosome 16
Location:7532785
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g683035 (1 of 2) IPR003593//IPR027417 - AAA+ ATPase domain // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCACATTGCCGTGTGGCCCAGCACCGCCACTACTACTGCTGCGACTGC
Internal bar code:CTTCGCCGGAGATGCAAAGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4374
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:75
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:75

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCCACTCAATGCCATGCT
Suggested primer 2:GTAGGTGAGGCCGTAGATGC