Insertion junction: CLIP2.049100_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.049100
Chromosome:chromosome 7
Location:1110554
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g320500 OPR29 OctotricoPeptide Repeat protein 29 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGTGGTTGCGAGCCACCAGGAACGCCAGCACCACACTGAGATCCAGCG
Internal bar code:GTGGCAGGCCAGTCACCACACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1484
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGCTGAATCGTCAATGG
Suggested primer 2:GCGCACACGTACAACAAGAG