| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049112 |
| Chromosome: | chromosome 6 |
| Location: | 3449340 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278103 | (1 of 4) 1.14.17.1 - Dopamine beta-monooxygenase / Dopamine beta-hydroxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGTCCGCTGCTCACCTTCCACAGCCGTATCAGTATGCCACCCATGTG |
| Internal bar code: | GAATCAAAGTTTGTTCCTCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3229 |
| LEAP-Seq percent confirming: | 77.5 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCACACACACGCGTTCTCT |
| Suggested primer 2: | TCTCCTAGCCAGTCATGCCT |