| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049116 |
| Chromosome: | chromosome 6 |
| Location: | 8332330 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307350 | (1 of 2) K12486 - stromal membrane-associated protein (SMAP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCAGCCAGCTGCTCGCATCTTACCATGGCTCATTACGCGTGGCAGCG |
| Internal bar code: | GGGTGCGGAGGGCGGTAATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 580 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAACTACCTGGGCCGAAT |
| Suggested primer 2: | GGCAAGGCTTTTTCAGTGGG |