| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049123 |
| Chromosome: | chromosome 8 |
| Location: | 2090258 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g369400 | (1 of 1) PTHR12356:SF18 - NUDC DOMAIN-CONTAINING PROTEIN 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGCGGCCTCCCCGCGCCTCTGCTTGCAGAGTGACCTGTTTGCGGCAG |
| Internal bar code: | AAACGGGGAAAAAGTGCAAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1647 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGCTGCAGATTCTTTAGC |
| Suggested primer 2: | TTCGCGTATTGGTTTGGGGA |