| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049126 |
| Chromosome: | chromosome 2 |
| Location: | 186450 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074370 | CDPK8 | Calcium/calmodulin-dependent protein kinase; (1 of 18) K13412 - calcium-dependent protein kinase (CPK) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCTGCTGTTGCCCACGCGGCTTGCTGAGGCTGTCCGGGGGCGAGTCG |
| Internal bar code: | TCTAAGCTCGACATAGGTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCTGGTGGCAAATGCAA |
| Suggested primer 2: | GGGACTCCTCATTGCTACCG |