| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049134 |
| Chromosome: | chromosome 3 |
| Location: | 3799820 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169800 | (1 of 2) PTHR10060:SF22 - CELL DEATH-RELATED NUCLEASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATGCGGTTGGGACATTGGTTTGGCTCGCTTGCTGGTCTCCGTTGTAG |
| Internal bar code: | AAGCTTAACTTCAGGGCTAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3386 |
| LEAP-Seq percent confirming: | 98.9247 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACAACGTTTGTGCTCTT |
| Suggested primer 2: | AGTGATGCCTATCTGACGCG |