Insertion junction: CLIP2.049153_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.049153
Chromosome:chromosome 8
Location:4252841
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGGACTGGAGTGGTTTCGGACGTTTGGCTCCTTCGATTACAACATGAC
Internal bar code:GGGGCAACAACTCATGAATTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2156
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTACTTGCAGACGACCGT
Suggested primer 2:GGACGACACTACAGGCCAAA