Insertion junction: CLIP2.049153_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.049153
Chromosome:chromosome 8
Location:4252847
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGAAACATACGTACCTTTCATACCCAAAACACCCCCTCTTGTAGCCTC
Internal bar code:GTCTTACATATTGTACTCGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1038
LEAP-Seq percent confirming:14.2857
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GGACGACACTACAGGCCAAA
Suggested primer 2:CCTTACTTGCAGACGACCGT