| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.049165 |
| Chromosome: | chromosome 12 |
| Location: | 2195648 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATGACGAAACCGATGGGTCGTAGTTGTATAAAAACTACAAGGGTGCGA |
| Internal bar code: | TATAAGTGTCCATTTGTGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4028 |
| LEAP-Seq percent confirming: | 98.4848 |
| LEAP-Seq n confirming: | 130 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 132 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCATGGAATGGTGAGCT |
| Suggested primer 2: | TGGCAAACATGTTCAAGGCG |