Insertion junction: CLIP2.049168_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.049168
Chromosome:chromosome 17
Location:295103
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g697950 AAA3 Plastidic ADP/ATP translocase; (1 of 2) PTHR31187:SF1 - ADP,ATP CARRIER PROTEIN 1, CHLOROPLASTIC-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTAGGTCCGCGGCGATTCCAGACGACTTTCGCCGGCTCTGCGTTCCCGA
Internal bar code:GGCTAGGGTAAGACATTGGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3553
LEAP-Seq percent confirming:84.7222
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTCAAAAACGCGAGCCCA
Suggested primer 2:CTACTGCACTCACACCAGCA