Insertion junction: CLIP2.049169_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.049169
Chromosome:chromosome 3
Location:3373702
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g166400 (1 of 2) IPR002591//IPR017849//IPR017850 - Type I phosphodiesterase/nucleotide pyrophosphatase/phosphate transferase // Alkaline phosphatase-like, alpha/beta/alpha // Alkaline-phosphatase-like, core domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACATCCCGCGCACCTGCTGCGCGCCCCTTCTCTTACTTGTTTTCCTACT
Internal bar code:AGCATATCATGAGTTTGGAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:270
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGGACTACTCACGATTCGGG
Suggested primer 2:TAAGCAATCGGCAGGCTTGA