| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049174 |
| Chromosome: | chromosome 3 |
| Location: | 1023704 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g148100 | (1 of 1) PTHR10281:SF4 - NEUFERRICIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAATGGCCGCCTTGCACCCTGCCACCACCCACAACAGACGTCGTGGTCA |
| Internal bar code: | GTACTTGAGTGGTCCGATCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2448 |
| LEAP-Seq percent confirming: | 97.1831 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACAGAAAACATGGCTGCA |
| Suggested primer 2: | ATGGCCTCCTCCTCACTCTT |