Insertion junction: CLIP2.049178_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.049178
Chromosome:chromosome 2
Location:7555341
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g393062 (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCGCCGCAAAACCACCAGCGCCTCCGCCTCCGCCGCGGTCACGCTTGT
Internal bar code:ATAACTCTTGTTCAATCACAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4654
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:GACGCGTATTTTCCTGTCGC
Suggested primer 2:AAATCCGCCACCTTGACGAT