| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.049193 |
| Chromosome: | chromosome 7 |
| Location: | 3616473 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337000 | CSD3 | (1 of 2) 5.1.1.17 - Isopenicillin-N epimerase; Putative cysteine desulfurase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGATACCACTGCTGCACCTCCAGAGCCAGCCTGACAGCGATCCAAA |
| Internal bar code: | GAAAATAAAATCTGTCCTGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1059 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACATGCGGTCGCGAATCT |
| Suggested primer 2: | GAGGTGTGTGTGTATGGGGG |