| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049194 |
| Chromosome: | chromosome 2 |
| Location: | 7494515 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392655 | (1 of 3) PF10358 - N-terminal C2 in EEIG1 and EHBP1 proteins (NT-C2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCCCGGCTGGCTATACATATTAGCGCGCGCATATCAATGATCAGCGAT |
| Internal bar code: | CTGCTTCTGAGTATTGCTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3106 |
| LEAP-Seq percent confirming: | 62.8571 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGATCGACGTGCAGGTT |
| Suggested primer 2: | GCTAGTTGCACACAAACGCA |