Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.049213 |
Chromosome: | chromosome 11 |
Location: | 3589785 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g478050 | (1 of 12) IPR000104//IPR002893 - Antifreeze protein, type I // Zinc finger, MYND-type | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATGTGTGGCCGGCGATGGTGGCTCTTTTGGTCTAGGCACGTCCCGGG |
Internal bar code: | CTCTTCGGTTTTTAAGTAGCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2079 |
LEAP-Seq percent confirming: | 94.7368 |
LEAP-Seq n confirming: | 18 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGCAAGACACACGGTTTCG |
Suggested primer 2: | TTCGTCATCACCAGCTGCAT |