Insertion junction: CLIP2.049226_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.049226
Chromosome:chromosome 17
Location:4234854
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g729600 (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTCCGCCCCAAACTCACGCGTCGTATTGCTCCGCCGCATCCTCCTCGC
Internal bar code:GATAGCTCAAATGGTGGGCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2466
LEAP-Seq percent confirming:82.3529
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCATCCCCTCAAACGCGA
Suggested primer 2:GGTCGACTGGGACTAGGACT