| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049246 |
| Chromosome: | chromosome 9 |
| Location: | 6540261 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415950 | PRPL6,uL6m,MRPL6 | (1 of 1) K02933 - large subunit ribosomal protein L6 (RP-L6, MRPL6, rplF); Mitochondrial/chloroplast ribosomal protein L10 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCCCAGCGGCGACCCCAGTCCGCGACCACACACACCTGCTCAATCTT |
| Internal bar code: | TGAATTTGCTACCTGAATCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 209 |
| LEAP-Seq percent confirming: | 18.1818 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCACTCGTGTTAAGCCC |
| Suggested primer 2: | CAGTGAGCAAACAAACCCCG |