Insertion junction: CLIP2.049250_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.049250
Chromosome:chromosome 2
Location:5779161
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g116900 SETB,HDP1 Homolog of small hydrophilic plant seed proteins; (1 of 1) PTHR34671:SF1 - EM-LIKE PROTEIN GEA6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACCTGGTATCCCTCGTGGCCCAGCTGCTCCTTCCTGCGCCCAGATC
Internal bar code:ACTTGATTTATCGCTGTCTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1986
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCATCCCAAAACCCATCA
Suggested primer 2:CGGGTAAGTATGGTCTCGGC