| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.049251 |
| Chromosome: | chromosome 10 |
| Location: | 3319864 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443300 | CNK5 | NimA-related protein kinase; (1 of 1) PTHR24361//PTHR24361:SF368 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTCGCTGGGCTGCGTGCTGTACGAGCTGTGCACACTCAGGTGGGCAC |
| Internal bar code: | ACGCGGGAAGGCTAATCTAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1028 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTGCACTCCAGCTGTAC |
| Suggested primer 2: | CCGTGAGTCCTTTGTCGACA |