Insertion junction: CLIP2.049254_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.049254
Chromosome:chromosome 6
Location:5010908
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g281700 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGAGAAACCGCAAGGACGTCAATCCAATCTGCAGACAGGCATACAGGGA
Internal bar code:GACCGGTTCAATCCCCTTTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1815
LEAP-Seq percent confirming:98.4127
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTTGTTGTGCTAACGCGG
Suggested primer 2:CCTCAAACAAGCACCACACG