| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049275 |
| Chromosome: | chromosome 9 |
| Location: | 2665969 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392000 | (1 of 3) PF09763 - Exocyst complex component Sec3 (Sec3_C) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATGCATGATTACGGGAGGGAGGGAGGCCGGGCGGGAGGCAGAGGGGC |
| Internal bar code: | TTTTAGATTTATGTCTCAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCCCAGACAAAAGAGACA |
| Suggested primer 2: | GTGTGTGTATGACCCCCTGG |