| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.049283 |
| Chromosome: | chromosome 17 |
| Location: | 4717008 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732900 | (1 of 1) IPR000104//IPR001126 - Antifreeze protein, type I // UmuC domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCCTCCCTACGCTGACCCACCTCGAGAGCTCCGGTGAAAAGAAGGTGT |
| Internal bar code: | CAGGACTAAGGGATTTTGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1311 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTAGGGGGTGGATTTGG |
| Suggested primer 2: | AACCTCTGCCGGTACCATTG |