| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.049284 |
| Chromosome: | chromosome 3 |
| Location: | 9194074 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g199423 | PYR4 | Dihydroorotase dehydrogenase; (1 of 1) 1.3.5.2 - Dihydroorotate dehydrogenase (quinone) / DHODH | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGATGTTTACAATCTACGGACATTACCGACAAGACCGACAAGGCTTA |
| Internal bar code: | GCTTCCGGACTTTGATTCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2282 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGATGTACGTGTGTCCACA |
| Suggested primer 2: | TTTTCCCTTCCGATCCCAGC |