Insertion junction: CLIP2.049289_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.049289
Chromosome:chromosome 1
Location:411128
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g002400 TGL1 (1 of 1) 3.1.1.3//3.1.1.73 - Triacylglycerol lipase / Triglyceride lipase // Feruloyl esterase / Hydroxycinnamoyl esterase; Putative triacylglycerol lipase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCACACGGGCGCTTTGTGGGAGGGGCTTTATTCGAGGCACCGACGGC
Internal bar code:ACCCTGACAAATTTGCTGGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2158
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:AATGAAGCGAAGGAGAGGCC
Suggested primer 2:ACCCTCCCTCCCTATCCCTA