Insertion junction: CLIP2.049295_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.049295
Chromosome:chromosome 2
Location:4966706
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g110450 (1 of 2) PF06201 - PITH domain (PITH); Proteasome-interacting thioredoxin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCTCCACTACTCTCCCCTCCCCTCCCCTCCCTGTACCTCAATATAGT
Internal bar code:CGCTTTCAGGGGGGACGCTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2344
LEAP-Seq percent confirming:98.0769
LEAP-Seq n confirming:51
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:AGGCTATCAGTGTCATCGCG
Suggested primer 2:CAGTGACAAGCTGCCTACCA